ABSTRACT
Abstract Background: IGF-1 may be an important factor in bone remodeling, but its mechanism of action on osteoclasts during orthodontic tooth movement is complex and unclear. Methodology: The closed-coil spring was placed between the left maxillary first molar and upper incisors with a force of 50 g to establish an orthodontic movement model. Eighty SD rats were randomized to receive phosphate buffer saline or 400 ng rhIGF-1 in the lateral buccal mucosa of the left maxillary first molar every two days. Tissue sections were stained for tartrate-resistant acidic phosphatase (TRAP), the number of TRAP-positive cells was estimated and tooth movement measured. Results: The rhIGF-1 group exhibited evidential bone resorption and lacuna appeared on the alveolar bone compared to the control group. Moreover, the number of osteoclasts in compression side of the periodontal ligament in the rhIGF-1 group peaked at day 4 (11.37±0.95 compared to 5.28±0.47 in the control group) after the orthodontic force was applied and was significantly higher than that of the control group (p<0.01). Furthermore, the distance of tooth movement in the rhIGF-1 group was significantly larger than that of the control group from day 4 to day 14 (p<0.01), suggesting that rhIGF-1 accelerated orthodontic tooth movement. Conclusion: Our study has showed that rhIGF-1 could stimulate the formation of osteoclasts in the periodontal ligament, and accelerate bone remodeling and orthodontic tooth movement.
Subject(s)
Humans , Animals , Rats , Osteoclasts , Tooth Movement Techniques , Periodontal Ligament , Insulin-Like Growth Factor I , Bone Remodeling , Rats, Sprague-DawleyABSTRACT
Objective:To explore the characteristics of local brain neural activity in post stroke aphasia (PSA) patients in different frequency bands and the relationship between Western Aphasia Battery (WAB) scores and specific frequency bands. Methods:From March, 2015 to May, 2018, 15 PSA patients, and 15 healthy adults as controls matched for age, gender and education were recruited. They were assessed with WAB and scaned with resting-state functional magnetic resonance imaging. The amplitude of low-frequency fluctuation (ALFF) and fractional amplitude of low-frequency fluctuation (fALFF) on the frequency bands of 0.01-0.08 Hz, 0.027-0.073 Hz, and 0.01-0.027 Hz were calculated. The ALFF and fALFF in different frequency bands were extracted and the correlation with the WAB scores in the patients were analyzed. Results:On 0.01-0.08 Hz, ALFF increased in the right precentral gyrus in the patients. On 0.027-0.073 Hz, ALFF increased values in the right precentral gyrus, and fALFF decreased in the right cerebellar Crus2 region; fALFF in the right cerebellar Crus2 region negatively correlated with the scores of information content (r = -0.576, P = 0.025), auditory comprehension (r = -0.658, P = 0.008), repetition (r = -0.616, P = 0.014) and aphasia quotient (r = -0.611, P = 0.016) of WAB. On 0.01-0.027 Hz, the fALFF decreased in the left inferior parietal limbic gyrus, and positively correlated with the scores of information content (r = 0.538, P = 0.039) and aphasia quotient (r = 0.526, P = 0.044). Conclusion:Resting-state fALFF abnormalities in PSA patients are frequency-dependent, which associate with some frequency-specific neurofunctional alterations.
ABSTRACT
To investigate the antitumor activity of shikonin against human colorectal cancer, the IC50 value towards four different human colon cancer cells was detected by MTT assay. In addition, a SW620 xenograft model was established and both the tumor volume and tumor inhibitory rate were calculated to evaluate the antitumor activity of shikonin in vivo. To further explore the mechanism of shikonin, metabolomics combined with multivariate statistical analysis was performed to analyse the profile of metabolites in mouse serum. The results show that shikonin can significantly inhibit the proliferation of four different colon cancer cell lines and exerted a high antitumor activity in vivo. The tumor inhibitory rate at low dose and high dose were 38.35% and 42.16%, respectively. In addition, a total of 38 potential biomarkers related to the antitumor effects of shikonin were identified through metabolomics analysis, including tryptophan, proline and methionine. The study revealed that the mechanism was related to disordered amino acid metabolism in colon cancer, especially in tryptophan metabolism. Our study suggests that shikonin could exert an antitumor effect by regulating amino acid metabolism in colon cancer and provides a theoretical foundation for further exploration and the eventual clinical application of shikonin.
ABSTRACT
The cognitive neuroscience researches about post-stroke aphasia provide the interpretation of all aspects of linguistics. The word-picture research paradigm can be applied to assess different types of aphasia, in various ways of stimulation modes and models. It is more helpful combining functional magenetic resonance imaging to research the mechanism of brain damage and recovery objectively. The interactive application of language task and imaging has also become a new direction in the mechanism study of aphasia.
ABSTRACT
OBJECTIVE@#To study the clinical features, treatment, and prognosis of pure red cell aplasia (PRCA) in children.@*METHODS@#A retrospective analysis was performed for the clinical data of 16 children with PRCA. The outcome and prognosis of patients treated with prednisone combined with Huaiqihuang granules versus prednisone alone were evaluated.@*RESULTS@#All the 16 children complained of symptoms of anemia including pale or sallow complexion. Of 12 children undergoing pathogen test, 7 (58%) were found to have pathogen infection, among which human cytomegalovirus was the most common. Lymphocyte subsets were measured for 7 children, among whom 5 (71%) had lymphocyte immune disorder. Six children were found to have abnormalities in immunoglobulin and complement. The 8 children treated with prednisone combined with Huaiqihuang granules had a median follow-up time of 21.5 months, among whom 1 was almost cured, 1 was relieved, and 6 were obviously improved; the median onset time of treatment was 1 month, and 2 children had disease recurrence in the course of drug reduction or withdrawal. The 8 children in the prednisone alone treatment group had a median follow-up time of 34 months, among whom 4 were almost cured, and 4 were obviously improved; the median onset time of treatment was 2.5 months, and 4 children had recurrence during drug reduction or withdrawal.@*CONCLUSIONS@#Children with PRCA usually complain of anemia-related symptoms. Laboratory tests show pathogen infection in some children with PRCA, and most of children have immune disorders. Glucocorticoids have a good therapeutic effect, but some children relapse in the course of drug reduction or withdrawal. Combined treatment with prednisone and Huaiqihuang granules may have a faster onset of action and less possibility of recurrence.
Subject(s)
Child , Humans , Glucocorticoids , Prednisone , Recurrence , Red-Cell Aplasia, Pure , Retrospective StudiesABSTRACT
Objective: To prepare nanobowls, establish the method for characterizing nanoparticles, and test drug loading efficiency and release efficiency of nanobowls. Methods: The polystyrene nanoparticles (PSNPs) were prepared by the means of the normal emulsion polymerization. The peanuts nanoparticles (PNPs) were synthesized by the swelling process of PSNPs and the selective crosslinking between 3-(trimethoxysilyl) propyl methacrylate and tetraethylorthosilicate. Finally, the polystyrene was dissolved to obtain nanobowls. Dynamic light scattering (DLS) was used to analyze the size of each kind of nanoparticles. Transmission electron microscope (TEM) was used to observe the morphology of nanoparticles. Nanobowls loaded doxorubicin hydrochloride as a model drug though continuous shaking were used to measure drug loading capacity and release efficiency. Results: PSNPs, coated polystyrene nanoparticles (CPSNPs), PNPs, silica peanuts nanoparticles (Si-PNPs) and nanobowls were synthesized successfully. The size of nanobowls was (126.7±4.9) nm and the Zeta potential was (-30.2±1.1) mV. The final nanoparitcles could be used to load drug easily. The drug loading efficiency and loading capacity reached 51.1% and 9.3%, respectively. Moreover, the nanobowls had the expected sustained release effect. Conclusion: The prepared nanobowls are loaded with drugs successfully, which can release drug slowly and almost completely. The nanobowls can be used for drug release delivery.
ABSTRACT
Objective To investigate the effect of the correction of the position error of the abdominal tumor by cone beam scanning. Methods Six patients with abdominal tumor were selected with double-blind method, and electronical portal imaing device was used to collect the information of the patient dosimetry during radiotherapy.EDOSE tool was applied to 3D dose reconstruction so as to execute real-time 3D dose verification of the treatment plan. Each patient had 5 sets of data acquired when image guidance existed and another 5 sets when image guidance was absent. The treatment effects with or without image guidance were compared.Results The overall γ passing rate with cone beam CT image guidance gained no advantages over that without image guidance, though the setup error was corrected to some extent. Image guidance contributed nothing to increasing target area coverage while decreasing exposure dose to normal tissue. Conclusion Setup error correction by the image guidance based on bone marker has no significant effect on improving 3D dose verification and dose accuracy during the radiotherapy of the abdominal tumor patient.
ABSTRACT
Objective· To prepare nanobowls,establish the method for characterizing nanoparticles,and test drug loading efficiency and release efficiency of nanobowls.Methods · The polystyrene nanoparticles (PSNPs) were prepared by the means of the normal emulsion polymerization.The peanuts nanoparticles (PNPs) were synthesized by the swelling process of PSNPs and the selective crosslinking between 3-(trimethoxysilyl) propyl methacrylate and tetraethylorthosilicate.Finally,the polystyrene was dissolved to obtain nanobowls.Dynamic light scattering (DLS) was used to analyze the size of each kind of nanoparticles.Transmission electron microscope (TEM) was used to observe the morphology of nanoparticles.Nanobowls loaded doxorubicin hydrochloride as a model drug though continuous shaking were used to measure drug loading capacity and release efficiency.Results· PSNPs,coated polystyrene nanoparticles (CPSNPs),PNPs,silica peanuts nanoparticles (Si-PNPs) and nanobowls were synthesized successfully.The size of nanobowls was (126.7±4.9) nm and the Zeta potential was (-30.2±1.1) mV.The final nanoparitcles could be used to load drug easily.The drug loading efficiency and loading capacity reached 51.1％ and 9.3％,respectively.Moreover,the nanobowls had the expected sustained release effect.Conclusion · The prepared nanobowls are loaded with drugs successfully,which can release drug slowly and almost completely.The nanobowls can be used for drug release delivery.
ABSTRACT
Dendrobium species on the ancient Chinese herbal texts were investigated in this paper, including their dscriptions of original species, producing areas and quality. Our results indicated that the major producing areas were Lu'an, Anhui province and Wenzhou, Taizhou, Zhejiang province. In addition, the sweet flavor, short, thin and solid stems were standing for good quality. Based on the stable producing areas and quality descriptions, D. catenatum (D. officinale) ("Tiepi Shihu") and D. houshanense were high quality medicinal Dendrobium species ("Shihu" ) in ancient China. Besides, there were 3 scientific names for "Tiepi Shihu", including D. candidum, D. officinale, D. catenatum. After textual investigation, We suggest that D. catenatum should be its scientific name, and D. officinale was synonyms published later. However, the name "D. officinale" could be reserved as it is much more popular used in publication and commodities. Moreover, its Chinese name should be "Tiepi Shihu".
ABSTRACT
Objective:To investigate the role of Notch signaling pathway in the migration of hepatocellular carcinoma cells and its related mechanisms.Methods:HepG2 was cultured in vitro,which was HL-7702,Huh-7.The migration ability of Transwell cells was detected,Notch,Snail,Westernblot to detect the expression of E-cadherin protein.1 mol/L DAPT and 5 mol/L DAPT were used to block the Notch signaling pathway,and compared the effects of different concentrations on cell migration and the expression of Snail and E-cadherin protein in hepatocellular carcinoma cells.Results:The invasion and migration ability of HCC cell lines was significantly higher than that of normal non neoplastic liver cells (P＜0.05).The invasion and migration ability of HepG2 was slightly higher than that of Huh-7,but the difference was not statistically significant (P＞0.05).Used Western blot method to detect protein imprinting cells Notch,SnaiI,E-cadherin protein expression,results showed that the expression of Notch and Snail in liver cancer cells than normal cells,the expression of E-cadherin was significantly lower than that of normal cells.Blocking the Notch signaling pathway by 1 mol/L DAPT and 5 mol/L DAPT results showed that compared with the control group,the expression of 1 mol/L DAPT and 5 mol/L DAPT can significantly reduce Snail,increased the expression of E-cadherin,and with the increase of the concentration of strengthening effect (P＜0.05).Conclusion:Notch signaling pathway plays an important role in the invasion and migration of hepatocellular carcinoma cells.It is believed that the expression of Snail and E-cadherin may be related to the expression.
ABSTRACT
Objective:To investigate the role of Notch signaling pathway in the migration of hepatocellular carcinoma cells and its related mechanisms.Methods:HepG2 was cultured in vitro,which was HL-7702,Huh-7.The migration ability of Transwell cells was detected,Notch,Snail,Westernblot to detect the expression of E-cadherin protein.1 mol/L DAPT and 5 mol/L DAPT were used to block the Notch signaling pathway,and compared the effects of different concentrations on cell migration and the expression of Snail and E-cadherin protein in hepatocellular carcinoma cells.Results:The invasion and migration ability of HCC cell lines was significantly higher than that of normal non neoplastic liver cells (P＜0.05).The invasion and migration ability of HepG2 was slightly higher than that of Huh-7,but the difference was not statistically significant (P＞0.05).Used Western blot method to detect protein imprinting cells Notch,SnaiI,E-cadherin protein expression,results showed that the expression of Notch and Snail in liver cancer cells than normal cells,the expression of E-cadherin was significantly lower than that of normal cells.Blocking the Notch signaling pathway by 1 mol/L DAPT and 5 mol/L DAPT results showed that compared with the control group,the expression of 1 mol/L DAPT and 5 mol/L DAPT can significantly reduce Snail,increased the expression of E-cadherin,and with the increase of the concentration of strengthening effect (P＜0.05).Conclusion:Notch signaling pathway plays an important role in the invasion and migration of hepatocellular carcinoma cells.It is believed that the expression of Snail and E-cadherin may be related to the expression.
ABSTRACT
An analytical method based on ultra performance liquid chromatography-tandem mass spectrometry was developed for the determination of SIOC0426 residues in environment samples ( soil, sediment and water) . The fate of SIOC0426 in three kinds of soils was also evaluated. The target compound was extracted with acetonitrile, cleaned up by C18 cartridge, and analyzed by gradient ultra performance liquid chromatography-tandem mass spectrometry with electrospray ionization under positive mode ( ESI+) using a UPLC BEH C18 column. The method was validated using fortified soil, sediment and water. Mean recoveries of SIOC0426 at three spiked levels ( 0 . 005 , 0 . 05 and 2 . 0 mg/kg ) ranged from 87% to 106% with relative standard deviations of 2. 8%-8. 0%. Limits of quantification ( LOQs) of SIOC0426 in soil ( sediment) and water were 0. 2 μg/kg and 0. 2 μg/L, respectively. Linear calibration functions with correlation coefficients R2>0. 9999 were obtained in the concentration range of 0. 5-20 μg/L. This method was applied to the analysis of SIOC0426 residues in soil degradation samples. The results showed that the half-life of SIOC0426 ranged from 1. 72 days to 28. 2 days in three kinds of soils under aerobiotic conditions. While, the half-life of SIOC0426 ranged from 2. 93 days to 31. 4 days in three kinds of soils under anaerobic conditions. The degradation speed of SIOC0426 under aerobiotic conditions was faster than that under anaerobic conditions in the same soil. And the degradation speed of SIOC0426 in soils may be connected with the pH value of soils, cation exchange capacity and soil texture.
ABSTRACT
DNA marker-assisted selection of medicinal plants is based on the DNA polymorphism, selects the DNA sequences related to the phenotypes such as high yields, superior quality, stress-resistance and so on according to the technologies of molecular hybridization, polymerase chain reaction and high-throughput sequencing, and assists the breeding of new cultivars. This study bred the first disease-resistant cultivar of notoginseng "Miaoxiang Kangqi 1" using the technology of DNA marker-assisted selection of medicinal plants and systematic breeding. The disease-resistant cultivar of notoginseng contained 12 special SNPs based on the analysis of Restriction-site Associated DNA Sequencing (RAD-Seq). Among the SNP (record_519688) was related to the root rot-resistant characteristics, which indicated this SNP could serve as genetic markers of disease-resistant cultivars and assist the systematic breeding. Compared to the conventional cultivated cultivars, the incidence rate of root-rot and rust-rot in notoginseng seedlings decreased by 83.6% and 71.8%, respectively. The incidence rate of root-rot respectively declined by 43.6% and 62.9% in notoginseng cultivation for 2 and 3 years compared with those of the conventional cultivated cultivars. Additionally, the potential disease-resistant groups were screened based on the relative SNP, and this model enlarged the target groups and advanced the breeding efficiency. DNA marker-assisted selection of medicinal plants accelerated the breeding and promotion of new cultivars, and guaranteed the healthy development of Chinese medicinal materials industry.
ABSTRACT
Objective To evaluated the safety and feasibility of excimer laser atherectomy (ELA) combined with drug-eluting balloon angioplasty in treating chronic ischemia of lower limbs.Methods ELA combined with paclitaxel-eluting balloon angioplasty was adopted to treat chronic ischemia of lower limbs caused by arteriosclerosis occlusive disease of lower extremity in three patients.All three patients had arteriosclerosis occlusive disease of superficial femoral artery;in two of them the disease was primary occlusive lesion and in another patient the disease was in-stent re-occlusion lesion after sten implantation.Results After the treatment,the blood flow in the diseased arteries was unobstructed,the blood supply of the lower limbs was obviously improved.No procedure-related complications occurred.Two weeks after the treatment,no recurrence of ischemic symptoms was observed,the blood flow in superficial femoral artery kept unobstructed.The patients recovered smoothly.Conclusion For the treatment of chronic ischemia of lower limbs,which are caused by the primary arteriosclerosis occlusive disease of lower extremity or by the in-stent re-occlusion lesion after sten implantation,ELA combined with paclitaxel-eluting balloon angioplasty is clinically safe and feasible,although its long-term effect needs to be clarified with more studies.
ABSTRACT
Objective To study balance function and surface electromyography (sEMG) activity of erector spinae (ES) and rectus ab-dominis(RA)in stroke patients.Methods From April,2015 to December,2016,17 stroke patients(test group)and 13 healthy subjects(con-trol group)were tested with balance platform:sitting with eyes open/close,with the platform swing of 10°,with trunk flexion and extension in maximum.The root mean square(RMS)of sEMG was recorded on both sides of ES and RA synchronously.The swing length and area of center of mass,the center of pressure on X and Y axes were also recorded as sitting with eyes open/close.Results For the test group,RMS of bilateral ES was significantly different as sitting with eyes open/close and with trunk flexion and extension in maximum (t>2.173, P<0.05).Compared with the control group,RMS of ES and RA in unaffected side increased as sitting with eyes open/close(t>2.175,P<0.05), as well as the swing length and area of center of mass(t>2.760,P<0.05).Conclusion The strength imbalance is found in ES in stroke pa-tients,which may associate with the balance function impairment,and need to be improved in rehabilitation.
ABSTRACT
An analytical method based on ultra performance liquid chromatography-tandem mass spectrometry was developed for the determination of SIOC0426 residues in environment samples ( soil, sediment and water) . The fate of SIOC0426 in three kinds of soils was also evaluated. The target compound was extracted with acetonitrile, cleaned up by C18 cartridge, and analyzed by gradient ultra performance liquid chromatography-tandem mass spectrometry with electrospray ionization under positive mode ( ESI+) using a UPLC BEH C18 column. The method was validated using fortified soil, sediment and water. Mean recoveries of SIOC0426 at three spiked levels ( 0 . 005 , 0 . 05 and 2 . 0 mg/kg ) ranged from 87% to 106% with relative standard deviations of 2. 8%-8. 0%. Limits of quantification ( LOQs) of SIOC0426 in soil ( sediment) and water were 0. 2 μg/kg and 0. 2 μg/L, respectively. Linear calibration functions with correlation coefficients R2>0. 9999 were obtained in the concentration range of 0. 5-20 μg/L. This method was applied to the analysis of SIOC0426 residues in soil degradation samples. The results showed that the half-life of SIOC0426 ranged from 1. 72 days to 28. 2 days in three kinds of soils under aerobiotic conditions. While, the half-life of SIOC0426 ranged from 2. 93 days to 31. 4 days in three kinds of soils under anaerobic conditions. The degradation speed of SIOC0426 under aerobiotic conditions was faster than that under anaerobic conditions in the same soil. And the degradation speed of SIOC0426 in soils may be connected with the pH value of soils, cation exchange capacity and soil texture.
ABSTRACT
DNA marked-assisted selection of medicinal plants accelerated the breeding and promotion of new cultivars, and guaranteed the healthy development of Chinese medicinal materials industry. The first disease-resistant cultivar of notoginseng, namely "Miaoxiang Kangqi 1", served as the object of study. We evaluated the Kangqi's resistance of seeds, seedlings and root against the pathological bacteria (Fusarum oxysporum) of root rot. Compared to the traditional cultivars, the disease index of notoginseng seeds declined by 52.0% after inoculation for seven days; the death rate of seedlings and disease index of root respectively decreased by 72.1% and 62.4% after inoculation for 25 days. Additionally, the growth inhibition ratio of notoginseng seeds and seedlings declined after inoculation. The seeds, seedlings and roots of "Miaoxiang Kangqi 1" showed significantly resistant to root rot. The evaluation of disease-resistance of Kangqi provided the basis for the popularization of new cultivar and guaranteed the favoring conduct of notoginseng pollution-free cultivation.
ABSTRACT
In view of the significant difficulties of propagation, planting and simple product in Dendrobium catenatum(D. officinale)industry development, a series of research were carried out. Genome study showed that D. catenatum is a specie of diploid with 38 chromosomes and 28 910 protein-coding genes. It was identified that specific genes accumulated in different organs at the transcriptome level. We got an insight into the gene regulation mechanism of the loss of the endospermous seed, the wide ecological adaptability and the synthesis of polysaccharides, which provided a theoretical basis for genetic engineering breeding and development and utilization of active pharmaceutical ingredients. The rapid propagation system was established for applying to industrialized production by overcoming breeding problems on seed setting and sprouting, which laid a foundation for artificial cultivation of D. catenatum. And in order to give a clear explanation of genetic variation of important economic traits, we built up the breeding system. Since special varieties of D. catenatum were bred, it helped solve the problem of trait segregation of seedling progeny and support the improvement of D. catenatum industry. The regulation of dynamic variation of target compounds, together with the mechanism of nutrient uptake, was revealed. The breakthrough of key technologies including culture substrates, light regulation and precisely collection was carried out. Several cultivation modes like facility cultivation, original ecological cultivation, cliff epiphytic cultivation, stereoscopic cultivation and potting cultivation were set up. Above all, the goal of cultivating D. catenatum as well as producing good D. catenatum will be achieved.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the specifificity of Tongli (HT 5) and Xuanzhong (GB 39) paired acupionts in aspects of Deqi sensation and brain activation patterns during electroacupuncture.</p><p><b>METHODS</b>In this study, 15 healthy subjects were enrolled. All participants suffered two kinds of functional magnetic resonance imaging (fMRI) examinations randomly: Examination A received electro-acupuncture (EA) at the bilateral Tongli (HT5) and Xuanzhong (GB 39) acupoints (ACU), and examination B received EA at bilateral non-acupoints (NAP). The subjects reported the feeling of Deqi at each examination later respectively. A multi-voxel pattern analysis method and Statistical Program for Social Sciences were used to analyze the data.</p><p><b>RESULTS</b>The ACU group (Exam A) reported fullness, heaviness, numbness, soreness and throbbing of signifificantly greater intensity than the NAP group (Exam B). In addition, there was no statistical signifificance between two groups in aching, tingling, deep pressure, sharp pain, dull pain, warmness and cold. Meanwhile, fMRI data revealed differences between two groups in discriminating accuracy of brain somatosensory cortex and language-related cortices.</p><p><b>CONCLUSIONS</b>Needling HT 5 and GB 39 may modulate language function through a complex brain network, suggesting that it may be benefificial to the recovery of language function in patients with aphasia.</p>
Subject(s)
Adult , Female , Humans , Male , Acupuncture Points , Brain , Pathology , Electroacupuncture , Methods , Magnetic Resonance Imaging , Pattern Recognition, PhysiologicalABSTRACT
Panax notoginseng is a commonly used traditional Chinese medicine with blood activating effect while has continuous cropping obstacle problem in planting process. In present study, a semimicroextraction method with water-saturated n-butanol on 0.1 g notoginseng sample was established with good repeatability (RSD<2.5%) and 9.6%-20.6% higher extraction efficiency of seven saponins than the conventional method. A total of 16 characteristic peaks were identified by LC-MS-IT-TOF, including eight 20(S)-protopanaxatriol (PPT) type saponins and eight 20(S)-protopanaxadiol (PPD) type saponins. The established method was utilized to evaluate the quality of notoginseng samples cultivated by manual intervened methods to overcome continuous cropping obstacles.As a result, HPLC fingerprint similarity, content of Fa and ratio of notoginsenoside K and notoginsenoside Fa (N-K/Fa) were found out to be as valuatable markers of the quality of samples in continuous cropping obstacle research, of which N-K/Fa could also be applied to the analysis of notoginseng samples with different growth years.Notoginseng samples with continuous cropping obstacle had HPLC fingerprint similarity lower than 0.87, in consistent with normal sample, and had significant lower content of notoginsenoside Fa and significant higher N-K/Fa (2.35-4.74) than normal group (0.45-1.33). All samples in the first group with manual intervention showed high similarity with normal group (>0.87), similar content of common peaks and N-K/Fa (0.42-2.06). The content of notoginsenoside K in the second group with manual intervention was higher than normal group. All samples except two displayed similarity higher than 0.87 and possessed content of 16 saponins close to normal group. The result showed that notoginseng samples with continuous cropping obstacle had lower quality than normal sample. And manual intervened methods could improve their quality in different levels.The method established in this study was simple, fast and accurate, and the markers may provide new guides for quality control in continuous cropping obstacle research of notoginseng.